Products and Services |ESA-blocking solution


Product Description

Bovine Serum Albumin-blocking solution (Catalog#: BSA-blocking) is 25% solutions of high-quality bovine serum albumin. It is suitable for Western blotting and immunohistochemistry (IHC). It is ready to use, no waiting for powder to dissolve with the concentrated formulation. As a single purified protein exists, BSA-blocking has fewer chances of cross-reaction with assay’s components than serum or milk solutions. BSA-blocking can also stabilize your antibodies and your protein of interests.  It is prepared in PBS, TBS, and proteinase-free water formulations to suit for a variety of applications.

 

Take 1 mL/0.4 mL of this BSA-blocking into 25 mL/10 mL of 1X PBST/TBST to make working blocking solution, or membrane blotting solution by adding your antibody into this 1%BSA-1X PBST/TBST solution.  Your primary antibody in this solution can be stored at 2-8°C by adding 0.1 % sodium azide (the final concentration, we have 100X stock, sodium azide stock can be provided for free when you order our BSA-blocking), and it can be used at least 5 times for membrane blotting.

 

Storage temperature: 2-8°C. 

 

For more information about BSA-blocking, please visit www.renovalife.com.

 

Western blotting using BSA-blocking_PBS. Western blot analysis of whole cell lysate from MCF-7 cells using RLI anti-beta-tubulin 01 antibody (A) and RLI anti-actin 01 (B). Primary antibody: (A) anti-beta-tubulin antibody: RLI, anti-beta-tubulin 01: 1:500; (B) RLI, anti-actin 01: 1:500. Secondary antibody: Goat anti-Rabbit IgG antibody, (H+L) Alkaline Phosphatase conjugate, Millipore, AP307A, 1:500. Expected size: beta-tubuling 55kDa shown by arrow (A); actin, 42 kDa, as shown by arrow (B).

 

Ordering Information

 

Cat # BSA-blocking_PBS:    100mL       $150.00 plus S&H


               

 

Cat # BSA-blocking_TBS:    100mL       $150.00 plus S&H


               

 

Cat # BSA-blocking_water: 100mL       $135.00 plus S&H


               

 

You can place your order by phone (904-379-6507) or fax (301-576-5078).
You can also send your inquires following this link.
Thank you for your business.


Product Flyer (pdf format)

 

Product Datasheet (pdf format)

 

Shipping and Return policy

Products and Services | ESA-blocking solution