Products and Services | Buffers for Pronuclear Injection of Zygotes-BAC/YAC DNA


Product Description:

Injection buffer is prepared for microinjection of BAC/YAC DNA into pronucleus of a zygote. Injection Buffer A contains 10 mM Tris, 0.1 mM EDTA in ultrapure ddH2O. Injection Buffer B contains 1000X polyamine mix. The pH value is adjusted to 7.4 with 1 M HCl, and then filtered 0.22 m filter. Animal embryos tested, and qualified.

 

Prior to use, add 1 L of Injection Buffer 2 (1000X polyamine mix) into 1 mL Injection Buffer 1. The ready-to-use buffer (with polyamine) should be prepared fresh for each experiment. Do not store the injection buffer when polyamine is added.

 

Catalog Number: RLI-MB-02

Applications: Mouse, rabbit, cattle zygotes

Sterility:Sterile-filtered

Unit: 1 mL/vial Buffer A, 10 L Buffer B

Quantity: 5 vials Buffer A, 1 vial Buffer B

Storage Temperature: Buffer A, 2-8 C; Buffer B, -20 C

Storage Duration: 3-6 months

Shipping : Wet Ice

 

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$75

Buffers for Pronuclear Injection of Zygotes-BAC/YAC DNA Cat #RLI-MB-02


 

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Products and Services | Buffers for Pronuclear Injection of Zygotes-BAC/YAC DNA