Products and Services | EZ-Zona Removal

Product Description:

EZ-Zona Remove kit (RLI-EZZR-01) is specially designed to remove the zona pellucida of embryos of mouse, rabbit and cattle. The kit contains two vials of Acidic Tyrode solution, two vials of pronase, and one vial of D-PBS-PVA for embryo washing. Pronase from Streptomyces griseus is prepared in M2 medium at a concentration of 5 mg/mL. All solutions are filtered 0.22 µm filter.  Animal embryos tested, and qualified.


To remove the zona pellucida, embryos are treated in acidic tyrode solution about 2-3 min under stereo-microscopy until the expanding and thinning of zona pellucida observed.  The embryos are then immediately transferred into pronase solution, and gently pipetted till all layer of zona pellucida is cleared off.  The embryos are naked and free of zona pellucida.  The embryos are finally washed either in D-PBS-PVA or directly in the culture medium prior to other use.


Catalog Number:                RLI-EZZR-01

Applications:                       Mouse, rabbit, cattle zygotes

Sterility:                               Sterile-filtered

Unit:                                      1 mL/vial

Quantity:                              2 vials Acidic Tyrode solution, 2 vials of pronase, 1 vial of D-PBS-PVA

Storage Temperature:     2-8 °C

Storage Duration:              3 months

Shipping :                             Wet Ice


Online Store
This item is available in the online store.
You can place your order by phone (904-379-6507) or fax (301-576-5078).
You can also send your inquires following following this link.
Thank you for your business.


EZ-Zona Removal Cat #RLI-EZZR-01


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Products and Services | EZ-Zona Removal